Abstract: Potato suspension cell culture system is particularly important in potato application research,which is the basis of potato breeding research and genetic engineering research.The construction of cell culture system can be roughly divided into four processes,namely,explant selection,callus culture,callus subculture,and suspension cell culture system construction.This paper focused on the establishment of potato suspension cell culture system and its application in the cultivation of new varieties,virus-free potato cultivation,the construction of potato germplasm gene bank and the application of biotransformation reactor.