Abstract: In order to promote the protection of Glycyrrhizagermplasm resources,using the roots of Glycyrrhiza uralensis of three years old from six producing areas as materials,glycyrrhizic acid was extracted by the hot reflux method and total flavonoids of Glycyrrhiza were extracted by the ultrasound-microwave-synergized extraction method,the contents of glycyrrhizic acid and glycyrrhizin were detected by UV spectrophotometry,the bacteriostatic test was carried out by drilling method,the content of effective components and antibacterial effect of Glycyrrhiza uralensis Fisch.from different producing areas were analyzed.The results showed that the content of glycyrrhizic acid in Qingyang Glycyrrhiza was the highest,which was significantly different from other regions except Bazhou,Xinjiang,and the content of glycyrrhizin was next to that in Xinjiang.Based on bacteriostatic experiment,the results showed that the licorice extract had a good inhibitory effect on Staphylococcus aureus,but a bad inhibitory effect on Salmonella,and the wild licorice from Qingyang had the best inhibitory effect.