Abstract:
In order to determine the tolerance value of different Elytrigia intermedia germplasms to NaCl, five samples of Elytrigia intermedia were used to simulate salt stress with different concentrations of NaCl solution(25, 50, 100, 200, 300, 400 mmol·L
-1). The germination rate, germination potential, germination index, vigor index, relative salt injury rate, and radicle length and plumule length of seedlings were measured, and the regression equation between relative germination rate and salt stress concentration was established.The results showed that with the increase of salt stress concentration, the germination index of seeds showed a downward trend, the relative salt damage rate showed an upward trend, and the radicle and embryo length also showed a downward trend, and the inhibition of salt stress on the radicle was greater than that on the embryo. The specific performance was that the low concentration NaCl(25 mmol·L
-1) stress had no significant effect on the germination rate, germination index, vigor index and embryo length of five germplasms. With the increase of salt stress concentration, the inhibition of seed germination increased. Under moderate NaCl(200 mmol·L
-1) stress, except Zhongyan 25, the germination rate of other four materials were more than 75%, and the germination potential of Zhongyan 25 seed was 0. Under the salt stress of high concentration(300 mmol·L
-1), the germination rate and germination index of five materials decreased significantly, and the relative salt damage rate increased significantly. Among them, Zhongyan 3 had the highest relative salt damage rate of 82.12%, and the Zhongyan 24 had lowest relative salt damage rate of 66.58%. When the salt concentration reached 400 mmol·L
-1, all materials did not germinate, and the relative salt damage rate reached 100%. According to the optimal one variable quadratic regression equation established, the salt tolerance of seeds at the germination stage was in the order of Zhongyan 13>Zhongyan 24>Zhongyan 25>Zhongyan 3>Zhongyan 1.