Abstract: In order to screen sea buckthorn varieties（lines）with specific functions for different purposes and explore functional seabuckthorn germplasm resources,this experiment took seven varieties（lines）of seabuckthorn of different subspecies as the test materials,and the main cultivar of Heilongjiang Province,Shenqiuhong,as the control,through comparative analysis of the basic characteristics of fruit,fruit oil,seed oil and total flavonoid content in fruit among different seabuckthorn subspecies.The differences in basic fruit traits,fruit oil,seed oil,and total flavonoid content in fruits among different seabuckthorn subspecies were analyzed.The results showed that the basic characters of the fruit were different among different varieties.The hybrid seabuckthorn fruit was small,the weight of 100fruits was small,but the fruit density was higher,the yield per plant was high,the fruit oil content and the total flavonoid content of the fruit were higher than those of the Mongolian seabuckthorn.The yield per plant of the Wanhuang seabuckthorn was 11.25kg,the oil content of the flesh was 37.93%,and the total flavonoid content was 2.27mg·g^-1,which was significantly higher than that of other seabuckthorn varieties.Its ripening period was late,the fruit did not wither in winter,and it was suitable for various harvesting methods.It is applicable to the production of processing varieties in the industrial application of seabuckthorn.The Mongolian seabuckthorn fruit was large in size,bright in color and large in weight,but the fruit density was low,and the yield per plant was lower than that of hybrid seabuckthorn,especially the Chuysk variety.Although the seed oil content was slightly higher than that of hybrid seabuckthorn,the premature ripening period was only suitable for manual picking.Therefore,in the application of seabuckthorn industry,it is suitable for sightseeing picking and fresh food processing varieties.