Abstract: In order to verify the application effect of microbial agents in the production of broomcorn millet, using the method of field test, Qishu 3 was used as the test material, and six microbial agents including Bacillus subtilis, Bacillus licheniformis, Trichoderma harziensis, Bacillus amyloliquefaciens, composite microbial agent and photosynthetic bacteria were irrigated in the root of the 5^th leaf stage, with no microbial agent as the control（CK）. Agronomic traits, yield traits, photosynthetic characteristics and soil enzyme activities of broomcorn millet under different treatments were analyzed. The results showed that compared with CK, the activities of soil urease, sucrase and neutral phosphatase were significantly enhanced after the application of microbial agent. The six microbial agents could significantly improve the agronomic traits（panicle length, effective tiller number, plant height, stem diameter）, yield traits（panicle weight, grain weight per panicle, yield） and photosynthetic characteristics（net photosynthetic rate, transpiration rate, intercellular CO₂ concentration） of Qishu 3, but had no significant effect on 1 000-grain weight. The results showed that the compound microbial agent and photosynthetic bacteria treatment had the best effect on improving soil enzyme activity and growth indexes of broomcorn millet, and the highest grain yield, respectively increasing by 14.90% and 13.45% compared with CK, which can be applied to the high-yield cultivation of broomcorn millet field in Heilongjiang Province.