Abstract: Plant tissue culture is the backbone course of agriculture and forestry, biological science, biotechnology and other majors in universities and technical schools, and also an important part of bioengineering technology. In order to train practical technical talents who can adapt to the development of modern horticultural production technology, the landscape architecture major of Hangzhou Xiaoshan Technician College takes plant tissue culture as an example, and optimizes the teaching mode according to the characteristics of the course and the problems in actual teaching. Based on the preparation of the sub task WPM basic culture medium of the plant tissue culture course, the five star teaching method is used to reform and innovate the teaching mode of the course. Effectively solve the problem of students′ low learning initiative and enthusiasm under the traditional teaching mode, let students′ theory and practice closely combine, ultimately improve students′ comprehensive quality and promote students′ all-round development, so that the output of talents in colleges and universities can meet the needs of today′s society for application-oriented and innovative talents.