Abstract: In order to select seabuckthorn strains with low fruit separation ability suitable for vibration harvesting, 10 different subspecies of seabuckthorn strains were used as experimental materials to measure related indicators such as branch fruit stem separation ability, fruit stem fruit separation ability, fruit stem length, and fruit hardness. The relationship between seabuckthorn branch fruit stem separation ability, fruit stem fruit separation ability, fruit stem length, and fruit hardness was explored. The results showed that among the 10 test materials, No.1-No.5 were subspecies of hybrid seabuckthorn between China and Mongolia. Among them, No.1-No.3 were small fruit lines with small, round or oblate fruits with yellow color. The fruit size and weight per hundred fruits were lower than those of medium and large fruits, and the average soluble solid content and fruit density were the highest. The materials No. 4 and No. 5 are medium fruit seabuckthorn varieties, with fruit volume, fruit weight, and fruit density between small and large fruit seabuckthorn varieties. The soluble solid content is similar to that of large fruit seabuckthorn varieties, and the fruit shape is similar to that of large fruit seabuckthorn varieties, with cylindrical or elliptical shape. The color is yellow and orange yellow, with a slightly sour flavor. The No.6 to No.10 varieties are Russian large fruit seabuckthorn, with fruit volume and average weight exceeding that of small and medium fruit seabuckthorn. The average fruit density of large fruit seabuckthorn is significantly lower than that of small and medium fruit seabuckthorn. The soluble solid solution is similar to the middle fruit type. The colors are divided into yellow, orange, and orange, with cylindrical and elliptical fruit shapes as the main ones. The average separation force between fruit branch and fruit stem（1.343 N） is greater than that between fruit stem and fruit（1.087 N）, and compared to the small fruit type sea buckthorn strain, the middle fruit type sea buckthorn strain has a closer fruit separation force to the large fruit sea buckthorn strain. The average values of fruit branch fruit stem separation force and fruit stem fruit separation force of the large fruit type seabuckthorn strain are higher than those of the small fruit type seabuckthorn strain. In the analysis of fruit handle and fruit hardness, as the fruit separation force of the test materials increases, the length of the fruit handle shows an increasing trend, while the fruit hardness shows a decreasing trend. Therefore, in the experiment, the separation force of small fruit shaped seabuckthorn is smaller than that of large fruit shaped seabuckthorn. However, in production, the vibration harvesting device used has a long stem of large fruit seabuckthorn, which has a good transmission effect on vibration. Therefore, the vibration harvesting effect is often better than that of small fruit shaped seabuckthorn.