Abstract: In order to clarify the effect of three-dimensional planting of Vitis amurensis Rupr.and Alliumvicto rialis L.on soil nutrients,this study compared the differences in organic matter,total nitrogen,alkali-hydrolyzed nitrogen,available phosphorus and available potassium,microbial quantity and enzyme activity in different soil depths.The results showed that compared with a single planting Vitis amurensis Rupr.or a single planting Alliumvicto rialis L.,three-dimensional planting could significantly increase the content of organic matter,total nitrogen,alkali-hydrolyzed nitrogen,available phosphorus and available potassium,quantity of bacteria,fungi and actinomycetes,and the activity of peroxide,urease,sucrase and phosphatase in each soil layer,and increased the output value per unit area.Therefore,choosing a reasonable three-dimensional planting method of Vitis amurensis Rupr.and Alliumvicto riadis L.can not only improvesoil utilization,but also improve the quality of the soil and increase comprehensive benefits.