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基于响应面法的杨树桑黄菌种液体培养基优化

Optimization of Sanghuangporus vaninii Liquid Culture Medium Based on Response Surface Methodology

  • 摘要: 杨树桑黄(Sanghuangporus vaninii)作为大型药用真菌,由于其生长年限长且开发不合理,导致其资源短缺,而通过液体发酵培养可以在短时间内得到大量菌丝体。本试验以菌丝体生物量为主要指标,对杨树桑黄液体培养基进行优化。通过响应面分析法对杨树桑黄液体培养基的碳源、氮源、无机盐进行筛选。结果表明:杨树桑黄ST菌株培养基的最佳碳源为葡萄糖,最佳无机盐为MgSO4·7H2O;最适培养基配方为葡萄糖34.44 g·L-1,蛋白胨7.26 g·L-1,酵母膏8.303 g·L-1,MgSO4·7H2O 2.618 g·L-1,pH自然;此时杨树桑黄生物量产量最高为2.733 05 g,与预测值基本一致,优化的液体培养基可以显著提高杨树桑黄ST菌丝生物量。

     

    Abstract: Sanghuangporus vaninii,a large-scale medicinal fungus, has a shortage of resources due to its long growth period and unreasonable development, and a large amount of mycelium can be obtained in a short time through liquid fermentation culture.In this experiment, the mycelial biomass was used as the main index to optimize the liquid medium of Sanghuangporus vaninii.Screening of carbon source, nitrogen source and inorganic salt in liquid medium of Sanghuangporus vaninii by response surface methodology.The results showed that the best carbon source of poplar mulberry was glucose.The best inorganic salt was MgSO4·7 H2O,the most suitable medium formula was glucose 34.44 g·L-1,peptone 7.26 g·L-1,yeast extract 8.303 g·L-1,MgSO4·7 H2O 2.618 g·L-1 and natural pH,at this condition, the biomass yield of Sanghuangporus vaninii was the highest at 2.733 05 g, which was basically consistent with the predicted value.It is concluded that the optimized liquid medium can significantly improve Sanghuangporus vaninii mycelial biomass.

     

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