Abstract: In order to promote the application of strawberry tissue culture seedlings in production and reduce production cost,the rooting method,seedling height,root length,shading rate,air humidity and ambient temperature on influence of domestication survival rate were analyzed by pot experiment in this study.The results showed that when the root length grew to 1-3 cm in culture room,the strawberry tissue culture seedlings should transfer to the acclimation room for continuation culture.When the seedlings grew to seedling height above 8 cm,root length above 4 cm,they should transplant to the hole tray of turfy soil.After transplantation,when shading rate was not higher than 40%,air humidity was not higher than 40%,and ambient temperature was 21-28℃,the survival rate of domestication could reach about 95%.