Abstract: In order to select the medium suitable for the breeding of Lilium varieties,in this experiment,the scales of Casablanca Liliumand Siberia Lilium were used as explants and inoculated on medium with different hormone concentrations.Through the induction,subculture,rooting,domestication and transplanting of adventitious buds of bulb,a systematic technique system of lily tissue culture and rapid propagation was established.The results showed that when the medium was MS+6-BA 2.0 mg·L^-1+NAA 0.1 mg·L^-1,the induction rate of adventitious buds in Siberia Lilium was the highest,76%,and the medium was MS+6-BA 2.0 mg·L^-1.The induction rate of the adventitious buds in the Casablanca Lilium was the highest,64%.When the medium was MS+6-BA 0.5 mg·L^-1+NAA 0.05 mg·L^-1,the proliferation rate of Siberia Lilium was the highest,which was 44%,When the medium was MS+6-BA 2 mg·L^-1+NAA 1.00 mg L-1,the proliferation rate of Casablanca Lilium was the highest,being 36%.The rooting effect of MS+NAA 0.5 mg·L^-1 was better.