Abstract: In order to improve the culture medium of potato virus-free seedlings,Fujin and Zhongshu No.5potato virus-free seedlings were used as test materials,and the concentrated liquid of Chinese herbal medicine was applied to the culture medium of potato virus-free seedlings to determine the effect of the concentrated liquid of Chinese herbal medicine on the growth of potato virus-free seedlings.The results showed that the addition of appropriate concentration of Chinese herbal medicine concentrate could increase the root length,leaf number and stem node number of potato virus-free seedlings,and shorten the stem node length.The fresh weight,number of tubers per bottle and the transplanting survival rate of virus-free test-tube seedlings were improved.Compared with the control,the root length of virus-free potato seedlings increased by 0.6cm,the number of leaves increased by 0.7-0.8leaves,the number of stem nodes increased by 1node,the length of stem node was shortened by 0.14-0.26cm,and the fresh weight of stem and leaf per plant of virus-free test tube plantlet increased by 0.04-0.06g,and the number of virus-free mini tubers increased by 3-4tubers per bottle.The survival rate of transplanting could be increased by 3.0%-4.5%.Moreover,after adding the concentrated liquid of Chinese herbal medicine,the pollution of fungi can be reduced and the time of hardening seedlings can be prolonged 3-4days in the process of hardening seedlings.