Abstract: To promote the factory seedling cultivation of raspberry,the raspberry varieties of"Double-season2381"and"Single-season 1132",introduced from Russia were used as experimental materials.The stem segment was used as explants.The orthogonal experiment was used to screen the best culture medium of axillary bud germination,cluster bud proliferation and tissue culture seedling rooting,so as to establish the technology system of rapid propagation.The results showed that the best medium of axillary bud germination"Double-season 2381"was 6-BA 1 mg·L^-1+NAA 0.3 mg·L^-1,and "Single-season 1132"was 6-BA 1.0 mg·L^-1+TDZ0.5 mg·L^-1+NAA 0.5 mg·L^-1,the germination rate was 91.7% and 92.3%,respectively,the growth rate of the axillary buds was fast and the leaf color was green.The best medium of cluster bud proliferation"Doubleseason 2381"was 6-BA 2.0 mg·L^-1+TDZ 0.5 mg·L^-1+NAA 0.1 mg·L^-1,"Single-season 1132"was 6-BA1.0 mg·L^-1+TDZ 1.0 mg·L^-1+NAA 0.3 mg·L^-1,the multiplication coefficients were 6.7 and 6.2,respectively,the growth rate of cluster buds was fast and the leaf color was green.The best medium of tissue culture seedling rooting"Double-season 2381"and "Single-season 1132"were 1/2 MS+IBA 0.2 mg·L^-1,the rooting rate was 96.9%and 95.1%,respectively,the rooting speed of tissue culture seedlings was fast and the leaf color was green.