生防菌MHZ111的鉴定及对黄瓜根结线虫病的防治效果评价

Identification of Biocontrol Strain MHZ111 and Its Control Effect Against Cucumber Root Knot Nematode

摘要: 为促进生防菌株的进一步开发利用，从漠河永冻层土壤中分离到菌株MHZ111,利用形态学和ITS、18S rDNA序列分析进行种类鉴定，并利用室内离体活性试验、盆栽和田间试验研究了该菌株对黄瓜根结线虫的防治效果。结果表明，菌株MHZ111为产紫青霉(Penicillium purpurogenum),该菌株发酵液原液对根结线虫二龄幼虫的校正死亡率达100%,在盆栽试验中防效达71.3%,在田间试验中防效达70.3%。产紫青霉MHZ111菌株在黄瓜根结线虫病的绿色防控上具有良好的开发应用前景。

Abstract: In order to promote the further development and utilization of biocontrol strains, strain MHZ111 was isolated from the permafrost soil in Mohe. The strain was identified by morphology, ITS and 18 S rDNA sequence analysis. Its control effect against cucumber root-knot nematode was evaluated through in vitro activity tests, pot and field experiments. The results showed that strain MHZ111 was identified as Penicillium purpurogenum. The relative mortality of the fermentation of the strain to Meloidogyne incognita J2 was as high as 100%,the biocontrol effect was 71.3% in pot experiment, and 70.3% in field experiment. Penicillium purpurogenum MHZ111 strain had a great potential for the green control of cucumber root knot nematode.