Abstract: Strawberry stem tip tissue culture and seedling cultivation techniques usually use three types of culture media of induction differentiation, proliferation, and rooting, and the process is relatively complicated. In order to explore a universal culture medium that can be used for both strawberry stem tip induction differentiation, proliferation, and rooting cultivation steps, and further simplify the operation process, the 0.3 mm stem tips of strawberry ’Red Face’ were used as test material, and growth hormones were used with 6-BA, TDZ, NAA, IAA and IBA at five different concentrations, and the orthogonal experiment of L₂₅（5~6） was applied. The general culture medium for inducing differentiation, proliferation, and rooting culture of stem tips was selected as MS+6-BA 0.3 mg · L^-1+TDZ 0.7 mg · L^-1+NAA 0.1 mg · L^-1+IAA 0.1 mg · L^-1+IBA 0.3 mg · L^-1. The induced differentiation rate was 67.2%, the value added coefficient was 8.5, and the rooting rate was 95.1%.