Abstract: In order to identify the presence of the resistance gene Sr31 in wheat materials the linked molecular markers SCSS30.2 and iag95 were used and to further determine the existence of the 1BL/1RS translocation in these materials. Using the PCR method, target fragments of 576 bp and 1100 bp were successfully amplified from positive control materials with SCSS30.2 and iag95, respectively. Among the 300 tested wheat materials, 10 varieties were detected with either the iag95 or SCSS30.2 molecular markers, with eight varieties carrying both markers. Using the ω-sec marker, we identified 9.67%, of materials possessing the 1BL/1RS translocation. Notably, all 8 materials identified as possibly containing the Sr31 gene were found to carry the 1BL/1RS translocation. Field disease resistance evaluations further revealed that, of the nine materials detected with the SCSS30.2 marker, all except Heichun 1 demonstrated strong field resistance. In contrast, the Nonglin 45, detected only with the iag95 marker, displayed susceptibility to both 21C3CTHQM and 34MKGQM pathotypes. In conclusion, this research successfully identified wheat materials carrying the Sr31 gene and the 1BL/1RS translocation, offering valuable molecular tools and germplasm resources for wheat resistance breeding.