Abstract: In order to promote the optimization and transformation of drug structure,293T cells were used for transfection and Western blot to study the role of viral hemagglutinin（HA）in endoplasmic reticulum（ER）stress response.The results showed that there were significant differences in the expression of HA proteins at different glycosylation sites.HA protein could induce ER stress response,and the expression of CHOP in different HA protein samples was different,which led to different apoptosis caused by ER stress response.