Abstract: In order to improve the culture medium of potato virus-free seedlings, Fujin and Zhongshu No.5 potato virus-free seedlings were used as test materials, and the concentrated liquid of Chinese herbal medicine was applied to the culture medium of potato virus-free seedlings to determine the effect of the concentrated liquid of Chinese herbal medicine on the growth of potato virus-free seedlings. The results showed that the addition of appropriate concentration of Chinese herbal medicine concentrate could increase the root length, leaf number and stem node number of potato virus-free seedlings, and shorten the stem node length. The fresh weight, number of tubers per bottle and the transplanting survival rate of virus-free test-tube seedlings were improved. Compared with the control, the root length of virus-free potato seedlings increased by 0.6 cm, the number of leaves increased by 0.7-0.8 leaves, the number of stem nodes increased by 1 node, the length of stem node was shortened by 0.14-0.26 cm, and the fresh weight of stem and leaf per plant of virus-free test tube plantlet increased by 0.04-0.06 g, and the number of virus-free mini tubers increased by 3-4 tubers per bottle. The survival rate of transplanting could be increased by 3.0%-4.5%. Moreover, after adding the concentrated liquid of Chinese herbal medicine, the pollution of fungi can be reduced and the time of hardening seedlings can be prolonged 3-4 days in the process of hardening seedlings.