Abstract: In order to develop and utilize raspberry resources more reasonably,the content of ellagic acid in the leaves,fruits and seeds of Heritage,Autumn Bliss and Porlka raspberries was determined by HPLC.The determination conditions were as follows,Agilent 18 chromatographic column（250 mm×4.6 mm,5μm）,injection volume 10μL,sampling time 45 min,flow rate 1.000 mL·min^-1,column temperature 30 ℃,and determination wavelength 254 nm.The results showed that there was a good linear relationship between the concentration of ellagic acid and the peak area in the range of 0.125-100μg·mL^-1,and the regression equation was y=0.017 3 x+1.623 5（R~2=0.998 5）.The content of ellagic acid was 16.41-1 364.99μg·g^-1.The content of ellagic acid was the highest in the leaves of Heritage and the lowest in the fruits of Porlka.The content of ellagic acid in different varieties of raspberry and different parts of the same variety was quite different.