Abstract: In order to further study the waterlogging resistance mechanism of Polygonatum sibiricum Red,we used 2-year-old Polygonatum sibiricum Red as experimental materials and treated in different degrees（mild,moderate and severe）of waterlogging at the beginning of flowering stage to discuss the effects of water stress on osmotic adjustment substance and antioxidant enzyme activities in 2-year-old Polygonatum sibiricum Red leaves.The results showed that the contents of proline and soluble protei and the activity of superoxide dismutase（SOD）,peroxidase（POD）and catalase（CAT）increased firstly and then decreased with the aggravation of waterlogging stress degree.All of them reached the lowest value under the condition of severe waterlogging stress except the activity of CAT.The content of soluble sugar increased with the aggravation of waterlogging stress degree.