Abstract: In order to select the suitable substrate for strawberry elevated cultivation and promote the recycling of resources,the effects of four different substrates on the growth and fruit quality of strawberry were studied.The results showed that after 40 days of strawberry transplanting,matrix CT（peat∶vermiculite∶perlite=2∶1∶1）and JZ（peat∶vermiculite∶perlite∶mushroom residue=5∶2∶2∶1）could significantly improve strawberry root activity,chlorophyll content,maximum single leaf area,plant height and stem diameter.Compared with CK（peat∶vermiculite∶vermiculite=3∶1∶1）,strawberry yield increased by 17.1% and 11.6% respectively.The content of soluble solids in CT treatment was the highest,which was 4.4% higher than that of CK.The VC content and sugar acid ratio in JZ treatment were the highest,which were 2.0%and 3.4% higher than that of CK,respectively.JZ treatment and CT treatment had better effect on the growth,fruit quality and yield increase of elevated strawberry,at the same time,it reduced the cost,and could be widely used in production.