Abstract: In order to improve the mutagenic effects of chemical mutagens,pingyangmycin(20,40 and80μg·mL^-1)were injected into the glume and sheath of two wheat varieties and made a direct effect on wheat gametes by husk and sheath injection.Agronomic traits of wheat genetic offspring was investigated.The results showed that in vivo mutagenesis treatment,the emergence rate of the M₁ of both Longfu 06 K508 and Longfu06-6798 decreased with the increase of concentration.There was a difference in the lethal dose between the two varied by glume injection,but the median lethal dose was not achieved by leaf sheath injection.The two treatments showed variation in plant height,growth period and awn type of M₁ and M₂.Data analysis showed that the plant height,ear length,and spikelet number of M₁ generation was significantly different to CK by glume injection method.However,the method of leaf sheath injection was not significant.Compared with CK,the ear length and spikelet number of the M₂ by leaf sheath injection method changed significantly,while the plant height and spikelet number of the glume injection method changed significantly and extremely significantly compared with CK.It indicated that the glume injection method has direct effect on male and female gametes,it was a good moisturizer and the effect time was long.It could produce abundant variations in mutagenesis progeny and be used in mutation breeding.