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不同剂量60Co-γ射线辐照对朝天椒种子发芽的影响

Effects of Different Doses of 60Co-γ Irradiation on Seed Germination of Capsicum frutescens

  • 摘要: 为进一步利用辐照诱变创制朝天椒种质资源和培育新品种,本研究采用不同剂量60Co-γ射线辐照朝天椒品种中农千斤红干种子、湿种子,探讨辐照对朝天椒种子萌发的影响。结果表明:低剂量辐照对朝天椒种子的发芽力(发芽势、发芽率)和发芽指数影响不明显,但随着辐照剂量的增加抑制作用逐渐增强,种子发芽力和发芽指数呈非线性逐渐降低的变化趋势,各处理均低于对照(0 Gy),干种子(25~3 000Gy)和湿种子(25~1 800Gy)的辐照剂量与种子发芽力和发芽指数间呈负相关。干、湿种子各在辐照剂量1 800和900Gy时,发芽率仅占供试种子数的一半,在1 800和3 000Gy时发芽率为0。种子逐日发芽率表现为先升后降、呈正态分布。湿种子发芽速率T50较干种子的上升速度快。干种子比湿种子具有更强的辐射耐受性。

     

    Abstract: In order to create new germplasm resources and breed new varieties of Capsicum frutescens by irradiation mutation,dry and wet seeds of Capsicum frutescens were irradiated with different doses of 60Co-γ,and explore the effect of irradiation on seed germination of Capsicum frutescens variety Zhongnongqianjinhong.The results showed that 60Co-γlow dose irradiation had no obvious effect on germination ability(germination potential,germination rate)and germination index,but with the increase of irradiation dose,the inhibition effect gradually increased,and the germination ability and germination index showed a nonlinear decreasing trend.Each treatment was lower than the control(0 Gy),and the radiation dose of dry seeds(25-3 000 Gy)and wet seeds(25-1 800 Gy)was negatively correlated with germination ability and germination index.The germination rate of dry and wet seeds was only half of the tested seeds at 1 800 Gy and 900 Gy,respectively.The germination rate was 0 at 1 800 Gy and 3 000 Gy,respectively.The daily germination rate of seeds increased first and then decreased,showing a trend of normal distribution curve.The germination rate T50 of wet seeds increased faster than that of dry seeds.Dry seeds showed stronger radiation tolerance than wet seeds.

     

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