Abstract: In this study,the stem segments with Dendrobium candidum node was used as the explant to study the best method of tissue culture for finding the optimal basic medium,optimal disinfection time,and studying the effect of different concentration of NAA and 6-BA to axillary-buds induction,clustered buds proliferation and rooting.The result showed that the best medium was 1/2MS,and the best sterilizing time of the explant of Dendrobium candidum with 1‰HgCl₂ was 10min.1/2MS+10g·L^-1 sucrose+0.6mg·L^-1 NAA+1.5mg·L^-16-BA was used as the optimal medium of axillary bud induction.The medium of proliferation of clustered buds was 1/2MS+0.6 mg·L^-1 NAA+1.5 mg·L^-1 6-BA.The optimal medium of inducing root was 1/2MS+0.5mg·L^-1IBA+0.9mg·L^-1 NAA.