基于SLAF-Super BSA技术的玉米萌发期耐冷性定位的初步研究
Preliminary Study on Location Cold Tolerance During Maize Germination Stage Based on SLAF-Super BSA Technology
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摘要: 为加快玉米萌发期耐冷性遗传育种研究,在对玉米重组自交系群体进行萌发期耐冷性鉴定的基础上,采用分离群体混合分析(Bulked Segregant Analysis,BSA)方法,对2亲本和2混池进行特异位点扩增片段测序(Specific-locus Amplified Fragment-sequencing,SLAF),初步定位了玉米萌发期耐冷性相关的染色体区段,并对关联区域内的基因进行初步富集分析。结果表明:研究获得了282 563个SLAF标签,每个SLAF标签平均父本测序深度为27.06×,母本平均测序深度为31.85×,耐冷池和冷敏感池的测序深度分别为36.72×和34.17×。获得多态性SLAF标签47 624个,多态性比例为16.85%。通过BSA关联分析在玉米第9号染色体上定位到了一个91.06Mb大小的玉米萌发期耐冷性关联区域,区域内共关联到3 170个基因。Abstract: In order to speed up maize genetics and breeding on cold tolerance during germination stage,Bulked Segregant Analysis（BSA）and Specific-locus Amplified Fragment-sequencing（SLAF）were used to locate the chromosomal regions related to the trait.The results showed that both two parents and two mixed pools were sequenced with SLAF method and 282 563 SLAF tags were obtained,the average paternal sequencing depth of SLAF tag was 27.06×and the average maternal sequencing depth of SLAF tag was 31.85×,sequencing depth of mixed pools were 36.72× and 34.17×respectively.47 624 polymorphism SLAF tags were obtained with a polymorphism rate of 16.85%.A 91.06 Mb region associated with cold tolerance during germination stage was located on chromosome 9 of maize,in which a total of 3 170 genes were associated.
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