Abstract: The social demand for innovative and entrepreneurial talents in biological majors is increasing rapidly with the rapid development of biological economy.It has become an inevitable trend to use the teaching concept of OBE to carry out research on the cultivation of students’ innovative and entrepreneurial ability.“Integration of specialty and innovation” is the key point of education reform in colleges and universities to cultivate high-level professional talents.Taking the biotechnology major of Daqing Normal University as an example, this paper proposes a curriculum construction system of “four stages and four dimensions”, and constructs a practical training system of “quaternity” and a service support system of “three levels of linkage” in order to solve the problems existing in the development of “professional innovation integration” in local colleges and universities. The concept of OBE education is highly compatible with the cultivation of innovation and entrepreneurship ability of college students in the new era, which plays a better role in promoting the cultivation of competitive high-quality talents for entrepreneurship and entrepreneurship in universities.