Abstract: In order to study the radiation mutagenic effect of different doses of ⁶⁰Co-dγ rays on foxtail millet seeds, Yugu 18 was used as experimental material.Foxtail millet seeds were irradiated with ⁶⁰Co-γ rays of 0,150,200,250,300 and 350 Gy to explore the effects of radiation on seed germination, seedling growth and physiological and biochemical indexes of foxtail millet. The results showed that Yugu 18 could significantly promote seed germination and seedling emergence at low radiation dose（150-200 Gy）. With the increase of radiation dose, seed germination and seedling growth are gradually inhibited, while 350 Gy dose will seriously damage foxtail millet seeds, seriously affect their seedling emergence and seedling growth, and the seedling emergence rate will drop sharply; With the increase of radiation dose, SOD activity and POD activity content in foxtail millet leaves showed a gradual upward trend, and SPAD value increased first and then decreased. According to the half lethal dose regression equation, the half lethal dose of Yugu 18 is 331.7 Gy.