Abstract: In order to explore the effect of suitable temperature and temperature on amylase activity and peroxidase activity of Clematis tangutica（Maxim.）Korsh.The germination, amylase activity and peroxidase activity of Clematis kansuensis seeds at different temperatures were determined.The result showed that: Clematis kansuensis seeds had a certain adaptability to low temperature, but the optimal germination temperature was 20-25 ℃.The germination time was delayed and the germination potential was decreased when the temperature was too high or too low.Low temperature had little effect on the activity of amylase and peroxidase, and the time to reach the peak value was only the difference of time.Under high temperature, the activity of amylase continued to rise.Although the activity of peroxidase increased slowly, the activity value was obviously low, and the germination rate was not high due to the accumulation of inhibitory substances.The highest germination rate was observed at 25 ℃,which was 76.6%.