Abstract: Experimental zoology is a comprehensive subject,rich in content and intersecting with many other subjects.Experimental zoology is a basic course for students majoring in veterinary medicine,and it is of great significance to comprehensively improve their subject cognition and experimental operation ability.This practical teaching activity took 65undergraduate students majoring in veterinary medicine of grade 2017as the object of teaching reform in College of Animal Science and Technology,Hainan University.In a complete course teaching process,the three-dimensional teaching method combining online and offline was adopted,and the effect of the reform method was evaluated by longitudinal comparison of questionnaire survey and final examination results.Through the real-time observation of the teaching process and the final quantitative data,the results show that the adoption of this three-dimensional teaching method can obviously stimulate students′initiative and enthusiasm in study,and significantly improve students′satisfaction with the course and performance.