Abstract: Oenothera biennis is a plant of Oenothera in Onagraceae, its dry roots or seeds can be used as medicine. Oenothera biennis is widely used for ornamental, edible, medicinal, industrial and other purposes. Heilongjiang Province is a cold region, with unique advantages in planting Oenothera biennis, and is one of the main planting and processing areas of Oenothera biennis. At present, there are problems such as low standardization and integration of agricultural technology, non-standard production management, etc. In order to better promote the planting technology of Oenothera biennis and improve the added value of products, this paper introduced the high-yield artificial cultivation technology of Oenothera biennis.It is expected to improve the yield, quality and product market competitiveness of Oenothera biennis and help the development of local characteristic agricultural industry, so as to promote the healthy and sustainable development of the characteristic Chinese herbal medicine industry in cold region.