Abstract:
In order to promote the artificial adjustment of the cultivation environment of Shatian pomelo,and explore the effects of different organic fertilizer treatments on soil bacterial community structure in Shatian pomelo orchard,this paper had a systematic study on the diversity and community structure changes of soil bacteria treated with five kinds of organic fertilizers by high-throughput sequencing technology,and then analyzed main components of this soil.Overall 51 383 optimized reads were obtained,Bacterial species detected in these samples covered 47 phyla,65 classes,145 orders,287 families,and 722 genera.According to diversity analysis,the results showed that dominant bacteria of soil bacteria at phyla level had slightly difference,among which Proteobacteria,Firmicutes,bacteroidetes,Chioroflexi and Actinobacteria were mainly dominant bacteria of soil in Shatian pomelo orchard.In terms of the genera level analysis,advantaged bacterial community has differed significantly in different samples.That being said,in the biogas residue processing samples,Streptococcus abundance was highest at 8.62%.In peanut bran processing samples,Chujaibacter abundance was the highest at17.97%.In the pig processing samples,Lactobacillus and Rhodanobacter had the highest abundance respectively with 7.83% and 5.26%.In terms of species level,it was found that the application of organic fertilizer reduced the relative abundance of Pseudomonas,while it increased the relative abundance of the potential probiotics Stenotrophomonas,among which the fecal organic fertilizer increased the relative abundance of three kinds of animal pathogenic bacteria.According to the redundancy analysis,it was found that 75.17% of bacterial community changes could be explained by selected environmental factors.In this study,it was suggested that the application of organic fertilizer should change the physical and chemical properties,the diversity and community composition of bacterial soil in Shatian pomelo orchard.In addition,it was proved that different organic fertilizers should have significantly different effects on the diversity and community composition of bacterial soil in Shatian pomelo orchard.