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秸秆与微生物菌肥配施对盐碱稻田土壤团聚体及真菌群落多样性的影响

Effects of Combined Application of Straw and Microbial Fertilizer on Soil Aggregates and Fungal Community Diversity of Rice Field Saline Alkali Soil

  • 摘要: 为改良培肥盐碱土,探索土壤团聚体和土壤真菌群落变化,采用盆栽试验,设置常规肥料处理,添加秸秆处理(9t·hm-2)及秸秆与微生物菌肥处理,于秋季水稻成熟期对土壤水稳性团聚体、土壤真菌群落和水稻产量进行测定,探讨秸秆与微生物菌肥配施对盐碱稻田土壤结构、土壤微生物和水稻产量的影响。结果表明,秸秆还田可降低土壤容重和pH,增加土壤有机质含量和土壤水稳性大团聚体数量,与常规施肥相比,添加秸秆后团聚体平均质量直径(MWD)可提高11.29%、添加秸秆和微生物菌肥后MWD可提高19.35%;秸秆和微生物菌肥对改良盐碱土物理和化学性状效果显著。Illumina MiSeq测序结果表明秸秆还田降低了土壤真菌Alpha多样性,但对子囊菌门和担子菌门丰度有促进作用;与常规施肥相比,添加秸秆可增加水稻产量,增产率为4.39%;添加秸秆和微生物菌肥同样能提高水稻产量,增产率为4.66%。建议进行秸秆还田的同时增施微生物菌肥,可对盐碱土改良起到积极作用,并且能增加水稻产量。

     

    Abstract: In order to improve and fertilize saline alkali soil and explore the changes of soil aggregates and soil fungal communities,pot experiments were conducted to determine the soil water stable aggregates,soil fungal communities and rice yield at the autumn rice maturity stage by setting up conventional fertilizer treatment,adding straw treatment(9t·ha-1)and straw and microbial bacterial fertilizer treatment,and to explore the effects of straw and microbial bacterial fertilizer on the soil structure,soil microorganisms and rice yield.The results showed that straw addition reduced the soil bulk density and pH,and increased the content of soil organic matter and the number of soil water-stable aggregates.Compared with conventional fertilization,MWD increased by 11.29%in the straw addition treatment,and MWD increased by 19.35%in the straw addition with microbial fertilizer treatment.Straw and microbial fertilizer have a significant effect on improving the physical and chemical properties of saline-alkali soil.The results of Illumina Miseq showed that straw addition treatment reduced the Alpha diversity of soil fungi,but promoted the abundance of Ascomycota and Basidiomycota.Compared with conventional fertilization,the straw addition treatment increased rice yield by4.39% and the straw addition with microbial fertilizer increased rice yield by 4.66%.It is suggested that straw addition with microbial fertilizer plays a positive role in saline soil improvement and this treatment is capable of increasing the rice yield.

     

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