Abstract: In order to explore the endophytic fungi resources of wild plants,endophytic fungi were isolated and purified from different tissues of 21 wild medicinal plants grown at the southern foot of the Daxing’an Mountains by tissue isolation method.Antagonistic strains were screened by plate confrontation method with common plant pathogens as target strains.The strains were identified according to colony morphology and molecular biology.The results showed that 61 antagonistic strains were screened out from 478 strains of endophytic fungi,among which 11 strains which inhibitory zone width greater than or equal to 5 mm were highly active.Among 61 strains,10 strains could not be identified by homology comparison,and the remaining 51 strains belonged to 16 genera.Among them,Penicillium accounted for 24.59%,Colletotrichum accounted for11.48%,Bipolaris,Phialophora,Alternariaand Fusariumaccounted for 6.56%,the rest were less than 5%.