Abstract: In order to select the suitable cultivation formula and improve the economic benefits,the mixture of bamboo and wood chips and straw was used as the main cultivation medium of Stropharia rugosoannulatafor comparative experiment.The results showed that using 50% bamboo chips and 50% wood chips and straw（1∶1）mixture to cultivate the mushroom,the yield was the highest,reached 5.6 kg·m^-2,the biological efficiency was 42%,the crude fat content was 2 times of the control group,and the input-output ratio was 0.63,the economic benefit was maximized.