Abstract: In order to promote the high quality and efficient production of rice in the Northeast region, this study used amino acid water-soluble fertilizer as the experimental material under the conventional planting mode of rice. The experiment was conducted by spraying amino acid water-soluble fertilizer on the seedling bed as the rice rejuvenation fertilizer. Four treatments and seven experimental points were set up to investigate their effects on the days of rice rejuvenation, seedling quality, and yield. The results showed that spraying 11.0 L·（667 m~2）^-1 of basic amino acid water-soluble fertilizer 2-3 days before transplanting resulted in an average of 6.9 days of rejuvenation, 3.1 days earlier than CK; 15 days after transplantation, the plant height increased by 13.9% compared to CK, the root length increased by 15.2% compared to CK, the fresh weight of the plant increased by 16.1% compared to CK, the fresh weight of the root increased by 19.7% compared to CK, the chlorophyll increased by 21.2% compared to CK, and the nitrogen content increased by 15.5% compared to CK; The yield increased by 6.3% compared to CK, and the net economic benefit increased by 89.62 yuan·（667 m~2）^-1compared to CK. At the same time, the data of amino acid water-soluble fertilizer+microbial inoculants and conventional fertilization treatment were improved compared to CK treatment, but the overall experimental data was weaker than that of amino acid water-soluble fertilizer treatment. Amino acid water-soluble fertilizer can reduce the number of days for rice to turn green, promote rice growth, and increase yield and economic benefits.