Optimization of Liquid Fermentation and Polysaccharide Extraction of Sanghuangporus sanghuang

Sanghuangporus sanghuang is a medicinal porous bacterium with good medicinal efficacy, and its polysaccharide is one of the main active ingredients, with antitumor, antioxidant, immunomodulatory and other pharmacological effects. In order to improve the dry weight of S. sanghuang mycelia and the yield of S.sanghuang polysaccharide, single factor and orthogonal test were used to optimize the medium formula and liquid fermentation culture conditions affecting the dry weight of S.sanghuang mycelia. Ultrasonic assisted extraction method was used to optimize the extraction conditions of polysaccharide yield of S.sanghuang. The results showed that the optimal medium formula of S. sanghuang was potatoes（peeled） 200 g·L^-1,lactose 32 g·L^-1, tryptone 10 g·L^-1, NaCl 2.00 g·L^-1, MgSO₄·7H₂O 1 g·L^-1, VB₁ 10.0 mg·L^-1, and mycelium dry weight was 2.82 g·L^-1. Under the conditions of temperature 28 ℃, inoculation amount 9%, rotational speed 180 r·min^-1 and pH6, the maximum mycelium amount was 3.12 g·L^-1. The optimal extraction process was as follows: ultrasonic power 200 W, solid-liquid ratio 1∶60, extraction time 40 min, temperature 50 ℃, the yield of polysaccharides was 5.54%. The results showed that the optimized culture medium formula, fermentation conditions and polysaccharide extraction technology of S. sanghuang were reasonable and feasible, which could provide reference for liquid fermentation of S. sanghuang mycelium to produce polysaccharide.