Establishment of Tissue Culture and Regeneration System of Young Spikes of Leymus chinensis

In order to optimize tissue culture and regeneration system of Leymus chinensis,six young panicles of Leymus chinensis were used as explants,and MS medium supplemented with different plant hormones was used to induce subculture differentiation of young panicles of Leymus chinensis,so as to establish an efficient regeneration system.The results showed that when the concentration of 2,4-D hormone was 5.0 mg·L^-1 in the induction medium,the induction rate of young panicles of the six new lines of Leymus chinensis were all high.The callus rate of Lc-6 was the highest（87.5%）,followed by Lc-4（81.5%）.The callus structure and state of the six strains were significantly improved by solid-liquid-solid（MS2-MSL2-MS2）subculture,the callus changed from loose to compact,the browning was significantly reduced,and the callus turned to milky white granules.The callus state of Lc-6 and Lc-4 was better than that of the other strains.The callus differentiation rate of the six strains on BNKT medium was significantly higher than that of the other two media（P＜0.05）,the differentiation rate of Lc-6 was the highest（41.25）;After transplantation,the survival rate of tissue culture seedlings of six strains was 100%.