Determination and Evaluation of Cyanoside Content in Flax Germplasm

In order to further evaluate the variation characteristics of the content of linamarin and lotaustralin in flax germplasm, this study was carried with 20 flax accessions and measure of high-performance liquid chromatography combined with evaporative luminescence detection determining the content of flaxseed linamarin and lotaustralin. The result showed that the average content of linamarin in flaxseed was 0.56±0.25 mg·kg^-1, and the coefficient of variation and genetic diversity index were 42.19% and 31.26% respectively.The average content of lotaustralin was 0.25±0.24 mg·kg^-1, and the coefficient of variation and genetic diversity index were 60.67% and 41.96% respectively. There was a significant positive correlation between the content of flaxseed linamarin and lotaustralin and total content. The correlation analysis between the content of flaxseed linamarin and lotaustralin and the yield and quality related traits of flaxseed showed a significant negative correlation（-0.133） between flaxseed linamarin and lotaustralin, but not significant with other fatty acid traits. Flaxseed linamarin and lotaustralin and total content were positive correlation significantly with plant height, and the correlation coefficient（0.216） with the total content of cyanogenic glycosides was the highest Clustering analysis showed while the genetic similarity coefficient value was 0.70 accessions was divided into three groups, and the average content of linamarin and lotaustralin in the first group was low, 0.46 mg·kg^-1 and 0.11 mg·kg^-1, respectively. Seven varieties of Neiya 6, H 140, Neiya 7, Neimenghong, CDC ARRAS, NOR MAN and Jinya 8 in this group could be widely used.