Abstract: In order to compare the difference of nutrient composition and hormone content of Danzhou chicken with different feather colored（flaxen feather or black feather）, the contents of routine nutrients, calcium and phosphorus of chest muscles and leg muscles were determined by using the methods recommended in the national food safety standards, the serum hormone content at the age of 1 week and 6 months was determined by using ELISA kit. The above determination results were compared and analyzed. The result showed that the conventional nutrients（crude ash, crude protein, crude fiber, crude fat） and the contents of calcium and phosphorus of flaxen feather Danzhou chicken were the same as black feather Danzhou chicken（P＞0.05）, but the difference was not significant. There was no significant difference in the contents of serum cholesterol and leptin between two different feather colored Danzhou chicken at 1-week-old and 6-month-old（P＞0.05）. At 1-week-old, the contents of lecithin and growth hormone of black feather Danzhou chicken was significant higher than that of flaxen feather Danzhou chicken（P＜0.01）. The content of estradiol was higher than that of flaxen feather Danzhou chicken（P＜0.05）. The contents of progesterone and insulin of black feather cock were significant higher than that of flaxen feather cock（P＜0.01）. At 6-month-old, the content of growth hormone of black feather Danzhou hen was significant higher than that of flaxen feather Danzhou hen（P＜0.01）. The results indicated that the nutritional value of two feather colors Danzhou chicken was similar, but the hormone content of the chicks was slightly different.