Abstract: In order to improve the utilization efficiency of boar semen in high temperature season in Yichun area, high motility sperm was obtained from boar semen by direct upstream method, filtration upstream method and self-made device upstream method in high temperature season. The quality of high-motility sperm obtained by the most suitable method was compared with that of ordinary sperm, and the effect of high-motility sperm and ordinary sperm on reproductive performance of sows was analyzed by combining with deep semen transfusion. The results showed that the filtration upstream method was the best method for the extraction of high-motility sperm and had the ability of re-dilution. The upstream treatment of diluted semen + deep semen transfusion could achieve higher birth rate and keep the pregnancy rate and delivery rate stable compared with the normal treatment of diluted semen + deep semen transfusion and the normal treatment of diluted semen + conventional semen transfusion. The results indicated that the upstream spermatozoa treatment plus deep semen transfusion could improve the semen utilization efficiency of boar in high temperature season.