Abstract: In order to explore the role of miR-802-5 p in the growth and development of sheep hair follicles, miR-802-5 p in sheep was obtained through high-throughput sequencing technology in the experiment, and its sequence conservation was analyzed. Target genes of miRNA were predicted by bioinformatics analysis methods, and GO analysis and KEGG pathway enrichment analysis was conducted for the acquired target gene set. The results showed that the expression level of miR-802-5 p in the two populations with and without hair removal showed an opposite trend, and the difference was the largest during the telogen phase of the hair follicle. A total of 994 target genes were obtained, among which FGF5, APC, SMAD4, and GSK3β were all genes related to hair follicle development or important influencing factors in Wnt related to hair follicle development. Target genes have shown that they play a role in the development of hair follicles and other biological processes, participate in the synthesis of PcG protein complexes, mitochondrial membranes and other cellular components, and involve the negative regulation of Wnt signaling pathways. The results indicated that miR-802-5 p may be a candidate miRNA affecting the growth stage of hair follicles.