Abstract: In order to confirm biological characteristics,serotype,virulence genes and drug resistance of pathogens cavsing fox and raccoon dog pnevmonia, pathogenic bacteria in this experiment were isolated from the lungs of pneumonia-obtained fox and raccoon dog in different farms in Heilongjiang Province and Shandong Province. Culture characteristics,PCR identification based on 16 S rRNA,serotype,virulence genes,animal pathogenicity and drug resistance of those pathogenic Escherichia coli strains were carried out. The results showed that all isolated 15 strains belonged to extraintestinal pathogenic Escherichia coli. The target gene fragments with a size of about 1 500 bp were amplified by PCR. The nucleotide sequence homology of 15 isolates was 99%,compared with the reference strains of Escherichia coli in GenBank. The serotypes were O_{112 ab} type containing 4 strains,O₈ type containing 3 strains, O₁₁ type containing 2 strains, and O₇₈,O₁₅₉,O₁₃₂,O₈₈,O₈₉, O₉ types containing one strain each,respectively. In 15 strains of extraintestinal pathogenic Escherichia coli, the detection rates of virulence genes such as fimC,yijp,tsh,ibeA,hlyF,ompT,fyuA,cva,ibeB,ompA,mat,iucD,colv and iroN were high,which were 60.0%-100%. The detection rates of other virulence genes were low,less than 27.0%. In animal pathogenicity test,mice were challenged with bacterial solution of 1×10~8 cfu/mL. The death occurred 12 hours post challenge,and the mice died successively from day 1 to 5,which suggested that the isolates showed strong pathogenicity to mice. The 15 strains of extraintestinal pathogenic Escherichia coli were 100% resistant to ampicillin,and those strains were highly resistant to piperacillin,nalidixic acid,ciprofloxacin,streptomycin,chloramphenicol,cefazolin,tetracycline,cinoxacin,aztreonam,kanamycin,levofloxacin and amikacin,which had drug resistance rates of 53.3%-93.3%. The results suggested that the pathogenic Escherichia coli from pneumonia-obtained fox and raccoon dog were extraintestinal pathogenic Escherichia coli. The serotypes were widely distributed. The isolated strains had strong pathogenicity and drug resistance.