Abstract: In order to study the effect of glutamine-enhanced parenteral nutrition on enteritis diseases, the experiment divided 6 rabbits into experimental group and control group at random, using 1% acetic acid and purified water enema respectively to establish the enteritis model. By observing the clinical symptoms and pathological changes to evaluate the establishment of the model, on the basis of the use of conventional symptomatic antimicrobial treatment, according to the different drugs added, the 24 established enteritis model rabbits were randomly divided into group Ⅰ（normal saline）,group Ⅱ（glutamine）,group Ⅲ（full price nutrient solution）,and group Ⅳ（glutamine and full price nutrient solution）; with 6 rabbits in each group. The clinical symptoms of rabbits were observed before treatment and 5 days after treatment; benzidine method was used to detect fecal occult blood; and disease activity index（DAI） was evaluated. Blood was collected for the measurement of blood routine and blood biochemical indexes with corresponding instruments. D-lactic acid（D-LA） content was detected by double-antibody one-step sandwich enzyme-linked immunosorbent assay（ELISA）. The results showed that compared with the control group, the experimental group had obvious clinical symptoms and pathological changes, and the rabbit enteritis model was successfully established. Compared with before treatment, DAI in group Ⅳ decreased significantly, which was lower than that in other groups（P＜0.05）. After treatment, the number of WBC in group Ⅳ was higher than that of other groups（P＜0.05）;the number of RBC and HGB in group Ⅲ and group Ⅳ were higher than those in groups Ⅰ and Ⅱ（P＜0.05）. Compared with before treatment, the number of PLT in each group increased after treatment, and group Ⅲ and group Ⅳ were significantly lower than group Ⅰ and group Ⅱ（P＜0.05）. The AST and ALT activities of each group were not significantly different before and after treatment（P＞0.05）. After treatment, the contents of ALB and TP in group Ⅰ were significantly lower than others Group（P＜0.05）;after treatment, the CRE and BUN contents of group Ⅲ and group Ⅳ were significantly higher than those of group Ⅰ and group Ⅱ（P＜0.05）,and the contents of BUN, CRE, TRIG and GLU of group Ⅲ and group Ⅳ were significantly higher than those of group Ⅰ and group Ⅱ（P＜0.05）. After treatment, the D-LA content of group Ⅳ was lower than that of other groups（P＜0.05）. The results suggested that glutamine-enhanced parenteral nutrition has the functions of reducing clinical symptoms, providing the body′s nutritional requirements, regulating immune function, and repairing the intestinal mucosal barrier, and could be used as an auxiliary treatment for enteritis diseases.