Abstract: In order to understand the carrier rate and drug resistance of Staphylococcus intermedius in dogs and cats in Yangzhou, 173 canine and 30 cat samples were collected from the Animal Hospital of Yangzhou University from September 2017 to June 2018. The Baird-Parker selective medium was used to isolate suspicious strains from the samples, and to identify whether the isolates were Staphylococcus intermedius,Gram stain microscopic examination and PCR amplification were carried out. The isolation rates of Staphylococcus intermedius in pets of different types, genders and ages were statistically analyzed. Finally, the drug susceptibility test was used to study the drug resistance of the isolated bacteria. The results showed that 32 strains of Staphylococcus intermedius were isolated from 203 samples, of which 31 strains of canine origin, accounting for 17.92%（31/173）,and 1 strain of cat origin, accounting for 3.33%（1/30）. The isolation rate of Staphylococcus intermedius was not significantly different in dog sex, age, and between dog and cat（P＞0.05）,but the risk of dog infection was 6.331 times that of cats（the ratio being 6.331,and the 95% confidence interval was 0.83-48.25）. Staphylococcus intermedius isolates had higher resistance rates to cotrimoxazole, kanamycin, doxycycline and amoxicillin, being 87.50%,65.63%,68.75% and 68.75%,respectively. 71.88%（23/32） of the isolates were multi-drug resistant bacteria, of which 1 strain of Staphylococcus intermedius was resistant to 7 types of antibiotics. The results suggested that the isolation rate and drug resistance rate of pet dog Staphylococcus intermedius from Yangzhou area were high, and most of them had multiple drug resistance.