Abstract: In order to study the polymorphism of the acid-sensing ion channel 1（ASIC1） gene in Springer Spaniels and its relationship with non-fear behavior trait, the polymorphism of ASIC1 gene in 166 Springer Spaniels was studied by DNA sequencing. The relationship between the ASIC1 gene and non-fear behavior trait was analyzed using the general linear model（GLM） in the software SPSS. The results showed that three single nucleotide polymorphic（SNP） sites in the exon 2, exon 4 and exon 5 of ASIC1 gene were found, named 9 G＞C,102 A＞C and 20 G＞A. 9 G＞C and 20 G＞A sites were moderately polymorphic, 102 A＞C site was low polymorphic. All these sites were not in the state of Hardy-Weinberg equilibrium. The non-fear behavior trait score of AC and CC genotypes of 102 A＞C site was significantly higher than that of AA genotype（P＜0.05）, but there was no significant difference between AC and CC genotypes（P＞0.05）. This results indicated that a SNP associated with non-fear behavior trait in Springer Spaniel was identified as molecular marker for early non-fear behavior trait, and could be used as a molecular marker assisted selection for early non-fear behavior trait in Spring Spanid.