Abstract: In order to diagnose the causes of rabbit disease in a rabbit farm in Pinghe Village, Matai Township and to find effective prevention and control measures, the experiment adopted the methods such as epidemiological investigation, clinical symptom observation, disease material smear microscopy, identification culture, biochemical identification were used to confirm the diagnosis of rabbit disease in the farm. Drug sensitivity test was carried out on the isolated pathogenic bacteria, and corresponding prevention and control measures were taken. The results showed that any isolation measures were not taken in the rabbit farm after the onset of the disease; the sick rabbits had the symptoms of fast onset, loss of appetite, diarrhea, and foul-smelling dark green feces. It can be seen by autopsy the stomach and intestines were full, the gastric mucosa had bleeding spots and ulcers, the small intestine was full of gas, and the intestinal wall was thin and translucent; the cecal cavity was full of gas and thin dark green content, the liver, spleen, and kidneys were dark brown, with liver texture becoming brittle. Gram positive fusobacteria with blunt round ends, single of cluster were found in the smear. No colony grew on ordinary agar medium or MacConkey medium for the diseased material, but black colonies grew on TSC medium. The isolate tested positive for glucose, fructose, maltose, gelatin, lactose and salicin, and tested negative for arabinose, sorbitol, ribose, mannitol, rhamnose and inositol. The isolate was highly sensitive to kanamycin, cefoperazone, sulbactam, amikacin and ceftiofur, and resistant to enrofloxacin, ciprofloxacin, erythromycin, cefazolin, etc. After disinfection and drug treatment, the rabbit farm’s situation was effectively controlled. The results suggested that the disease of rabbits in this farm was caused by rabbit Clostridium perfringens,and the isolated Clostridium perfringens was resistant to some antibiotics.