Abstract: In order to understand the prevalence and genetic variability of Porcine epidemic diarrhea virus（PEDV） in Hebei region in recent years, this experiment was conducted to collect small intestinal tissues of piglets from a pig farm in Hebei Province, for extracting RNA,performing RT-PCR based on S1 gene, cloning and sequencing of amplified positive products, homology analysis of sequencing results, and the analysis of hydrophilicity, surface palpability, antigenic indicators and T cell motifs of S1 protein. MEGA X 10.0.5 software was used to perform genetic evolution analysis of PEDV S1 gene using maximum likelihood（ML）. RDP4 software was used to carry out the recombination analysis of PEDV S1 gene. The results showed that pMD18-T-PEDV-S1 was successfully cloned, and the strain was named HB/HS/2019 strain, which contained the nearly full-length sequence of the S1 gene with the N-terminal domain and the C-terminal domain, and the length was 2 163 bp, encoding 721 amino acids; potential B cell epitopes and T cell motifs of S1 were successfully predicted. After homology analysis, HB/HS/2019 had the highest gentic similarity with Sichuan strain CH/SCJY/2018 S1 gene, which was 99.8%,and 91.4% gentic similarity with Belgium CV777 strain, with insertions and deletions. According to evolutionary analysis, 40 PEDV strains were divided into G Ⅰ group and G Ⅱ group; HB/HS/2019 belonged to G Ⅱ group, and in G Ⅱ group, all epidemic strains appeared in China after 2010;HB/HS/2019 was closely related to the commercially available vaccine strains AJ1102 and LW/L,and relatively distant to the CV777 and attenuated CV777 strains. After recombination analysis, no potential recombination events were found in the HB/HS/2019 S1 sequence, and there were 3 recombination events in the S1 gene between the strains of G Ⅰ group and G Ⅱ group. The results suggested that the HB/HS/2019 S1 gene was closely related to the mutant strains reported in China after 2010;although there were some nucleic acid site mutations in this gene, no genetic recombination event had occurred yet.