Abstract: To investigate the effects of compound enzyme preparation on nutrients apparent digestibility and hepatic metabolizing of chickens fed a low protein diet, a total of 240 one-day-old commercial broilers were randomly divided into six groups in this study. Chickens in the control group was fed a basic diets, and chickens in the experimental groups Ⅰ-Ⅴ were fed base diets with different protein levels（0, 0.5%, 1.0%, 1.5%, 2.0%） and 0.1% compound enzyme preparation, respectively. After feeding for 42 days, feces and serum were collected and used to detect the apparent nutrient digestibility of calcium（Ca）, phosphorus（P） and crude protein（CP）, as well as the activities of Glutamic-pyruvic transaminas（GPT）, Glutamic oxaloacetic transaminase（GOT） and Alkaline phosphatase（ALP）. The results showed that the apparent digestibility of Ca, P, CPs as well as ALP in group Ⅰ was significantly higher than that in the control group（P＜0.05）, and ALP activity in serum was significantly increased（P＜0.05）, but the activities of GPT and GOT were significantly lower（P＜0.05）. Compared with group Ⅰ, the apparent digestibility of Ca and P in group Ⅱ-Ⅴ showed a downward trend, but Ca and P in group Ⅱ-Ⅲ still significantly higher than that in control group（P＜0.05）. The apparent nutrient digestibility of CP showed an upward trend. In addition, the GOT activity in group Ⅲ was significantly decreased compared with control group（P＜0.05）, and ALP activity was significantly increased（P＜0.05）. The results indicated that the basal diet supplemented with 0.1% complex enzyme preparation while reducing 1.0% protein content could still improve the liver metabolism of broilers and increase the utilization rate of feed digestion, thus appropriately reducing the production cost.