Abstract: In order to study the influence of keratin associated protein（KAP） 3.2 gene on the economic characters of semi-coarse-wool sheep wool, the total RNA was extracted from skin hair follicles of plain Hetian sheep, a local breed in southern Xinjiang Province, and cDNA was obtained by reverse transcription and PCR amplification was performed. The recombinant clone plasmid pMD18-T-KAP3.2 and the recombinant expression plasmid pET-28 a-KAP3.2 were constructed, and bioinformatics software was used to analyze the gene. The results showed that KAP3.2 gene of plain Hetian sheep hair follicle was successfully cloned, the gene fragment length was 306 bp, encoding 98 amino acids. The sequence of this gene had 98.7% homology with the nucleotide sequence of Merino sheep（M21099.1） in GenBank. There were four mutations, namely, the mutation of G at position 104 was C, the mutation of C at position 138 was T, the mutation of C at position 226 was G, and the mutation of C at position 265 was G. Through translation comparison, there were three mutations in the amino acid sequence encoded by the KAP3.2 gene of plain Hetian sheep. The amino acid sequence was mutated from serine to threonine at position 35, from arginine to glycine at position 76 and 89.The results indicated that KAP3.2 gene sequence of plain Hetian sheep hair follicle was highly conserved although some bases were changed. KAP3.2 gene sequence was homologous and closely related to Merino sheep in GenBank.