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添加杜仲粉对花生秧发酵CNCPS中蛋白和碳水化合物组分的影响

Effects of Eucommia ulmoides powder on protein and carbohydrate components in CNCPS during peanut vine fermentation

  • 摘要: 为了探究添加杜仲粉对发酵花生秧的感官评定、康奈尔净碳水化合物和蛋白质体系(CNCPS)中蛋白及碳水化合物组分的影响,试验分别设置未发酵组、对照组(仅添加混合菌液发酵)、1%杜仲粉组(添加1%杜仲粉和混合菌液发酵)、3%杜仲粉组(添加3%杜仲粉和混合菌液发酵),每组5个重复,将乳酸菌、枯草芽孢杆菌和地衣芽孢杆菌3种菌等量混合,添加比例为发酵料干物质的5%,各组水分含量为55%,在室温(18~22℃)条件下,发酵袋厌氧发酵45 d后开封,对发酵花生秧进行感官评定,并分析测定其营养成分,计算饲料蛋白组分非蛋白氮(PA)、快速降解蛋白(PB1)、中速降解蛋白(PB2)、慢速降解蛋白(PB3)和结合蛋白(PC)和碳水化合物组分总碳水化合物(CHO)、快速降解碳水化合物(CA)、中速降解碳水化合物(CB1)、缓慢降解碳水化合物(CB2)、非结构性碳水化合物(CNSC)和不可利用碳水化合物(CC)含量。结果表明:在添加杜仲粉微贮发酵后,花生秧颜色更接近于原料,质地和气味更优于未添加组,发酵后的花生秧感官评定质量均达到优良等级。与对照组比较,3%杜仲粉组PB1含量显著增加(P<0.05),而PA和PC含量显著降低(P<0.05),且3%杜仲粉组PB1含量显著高于1%杜仲粉组(P<0.05),PA含量显著低于1%杜仲粉组(P<0.05),但PC含量差异不显著(P>0.05);1%和3%杜仲粉组的CHO、CA、CB1、CB2和CNSC含量与未发酵组和对照组比均差异不显著(P>0.05),仅CC含量显著降低(P<0.05)。说明在发酵过程中添加一定量的杜仲粉能够改善花生秧的品质和营养价值。

     

    Abstract: To investigate the effects of Eucommia ulmoides powder on the sensory evaluation, the Cornell net carbohydrate and protein system(CNCPS)of fermented peanut vine, the experiment was divided into four groups: unfermented group, control group(fermentation by adding mixed bacterial solution only), 1% Eucommia ulmoides powder group(fermentation by adding 1% Eucommia ulmoides powder and mixed bacterial solution) and 3% Eucommia ulmoides powder group(fermentation by adding 3% Eucommia ulmoides powder and mixed bacterial solution),each group had 5 replicates. Lactic acid bacteria, Bacillus subtilis and Bacillus licheniformis were mixed in equal amount, and the proportion was 5% of dry matter of fermentation material. The water content of each group was 55%. After anaerobic fermentation at room temperature(18-22 ℃) for 45 days, sensory evaluation was carried out on fermented peanut vine, protein components (non-protein nitrogen(PA), rapidly degraded protein(PB1), intermediately degraded protein(PB2), slow degraded protein(PB3) and conjugated protein(PC) and carbohydrate componentstotal carbohydrate(CHO), rapidly degraded carbohydrate(CA), intermediately degraded carbohydrate(CB1), slow degraded carbohydrate(CB2), non-structural carbohydrate(CNSC) and unavailable carbohydrate(CC) were determined and analyzed. The results showed that the color of peanut vine was more similar to raw material, the texture and smell of peanut vine were better than those of the non-add group, the sensory evaluation quality of peanut vine reached the excellent grade after adding Eucommia ulmoides powder microsilage for fermentation. Compared with the control group, the content of PB1 in 3% Eucommia ulmoides powder group was significantly increased(P<0.05), while the contents of PA and PC were significantly decreased(P<0.05). The content of PB1 in 3% Eucommia ulmoides powder group was significantly higher than that in 1% Eucommia ulmoides powder group(P<0.05), and the content of PA was significantly lower than that in 1% Eucommia ulmoides powder group(P<0.05),but there was no significant difference in PC content(P>0.05). The contents of CHO, CA, CB1, CB2 and CNSC of fermented peanut vine in 1% and 3% Eucommia ulmoides powder groups were not significantly different(P>0.05) from those in the un-fermented and control groups, but the content of CC was significantly reduced(P<0.05). The results indicated that adding certain amount of Eucommia ulmoides powder during fermentation could effectively improve the quality and nutritional value of peanut vine.

     

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